Faculty Members

Dr. Debabrata Mandal
Assistant Professor
Department of Biotechnology

National Institute of Pharmaceutical Education & Research,
(NIPER), Hajipur-844 101, Bihar, India.

Phone No. : +91-7091231863, +91-8335996348
E-mail : debabrataman@gmail.com, debman_76@yahoo.co.in

Research Interest and projects:

My research group is looking for new vaccine candidates against the disease visceral leishmaniasis (VL) caused by the parasite Leishmania donovani. Leishmania species are causative agents of a parasitic infection that manifests itself in a variety of clinical forms depending on the species of Leishmania and the immunological status of the host. Leishmania donovani is the etiological agent of Kala-azar, a chronic and often fatal form of human visceral VL. Over more than 90% cases of VL ensue in 5 countries: India, Bangladesh, Nepal, Sudan and Brazil. My group is also looking for new chemotherapeutic agents targeting the ergosterol (a lipid that is unique to parasite/microbes but absent in human) biosynthesis pathway of leishmania. Current Research projects are:

Mechanism of anti-leishmanial effect mediated by medium chain fatty acids and their derivatives
Cloning, Purification and characterization of initiation factor 4a and sterol methyltransferase of Leishmania donovani as potential vaccine candidates
Application of gold nanoparticles for detection of SAG-resistant strains using single nucleotide polymorphisms and over- expressed genes as biomarkers

Teaching Interests:
Department of Biotechnology at NIPER-Hajipur offers multiple courses that perfectly amalgamate the interest of multiple disciplines including Biochemistry, Microbiology, Immunology, Molecular Biology and Pharmaceutical sciences. I teach courses on Biochemical engineering, Spectroscopy, Immunology, protein structure and stability etc.
Educational qualifications:

Ph.D. (1999-2005) at Bose Institute, Kolkata (Thesis title---New insights into the mechanisms governing structure and function of human erythrocyte membrane)

M. Sc. in Biotechnology (1996-1998) from IIT-Mumbai (1st class)

B.Sc. in Chemistry (Honours) from University of Calcutta (1993-1996), Ramakrishna Mission Residential College-Narendrapur (1st Class)


Professional experience:

Assistant Professor in Biotechnology at NIPER- Hajipur (August 2014- till date)

Scientist II at Vyome Sciences Private Limited, Delhi (2012- 2014)

Research Scientist at Massachusetts Institute of Technology (MIT), Dept. of Biology (2006-2012)
(Project title —Role of tRNA modifications in codon-anticodon interaction during protein translation)
Research publications:

Bhattacharya, A., Köhrer, C., Mandal, D. & RajBhandary, U. (2015). Nonsense Suppression in Archaea. PNAS 112, 6015-6020.

Mandal, D., Kohrer, C., Su, D., Ramesh Babu, I., Chan, C.L.T., Liu, Y., Soll, D., Blum, P., Kuwahara, M., Dedon, P.C. & RajBhandary, U.L. (2014). Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs. RNA 20, 177-188.

Kohrer, C., Mandal, D., Gaston, K.W., Grosjean, H., Limbach, P.A. & RajBhandary, U.L. (2014). Life Without tRNAIle-Lysidine synthetase; translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical-tRNA2Ile. Nucleic Acid Research. 42(3), 1904-1915.

Voorhees, R., Mandal, D., Neubauer, C., Köhrer, C., RajBhandary, U.L. and Ramakrishnan, V. (2013).The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Nature Struc. & Mol. Biology. May; 20(5): 641–643.

Mandal D, Kohrer C, Su D, Russel, SP, Krivos, K, Castleberry CM, Blum P, Limbach PA, Soll, D and Rajbhandary UL (2010). Agmatidine, a modified cytidine on the anticodon of archaeal tRNAIle, base pairs with adenosine but not with guanoshine. PNAS, 107(7), 2872-2877.

Köhrer C, Srinivasan G, Mandal D, Mallick B, Ghosh Z, Chakrabarti J and Rajbhandary UL (2008). Identification and characterization of a tRNA decoding the rare AUA codon in Haloarcula marismortui. RNA, 14 (1), 117-126.

Singh, A. K., Haldar, R, Mandal, D. and Kundu, M. (2006). Analysis of the topology of Vibrio cholerae NorM and identification of amino acid residues involved in Norfloxacin resistance. Antimicrob. Agents & Chemother. 50(11), 3717-3723.

Mandal, D., Mazumder, A., Das, P., Kundu, M. and Basu, J. (2005) Fas-, caspase-8, and caspase 3-dependent signaling regulates the activity of aminophospholipid translocase and phosphatidylserine externalization in human erythrocytes. J. Biol. Chem. 280(47), 39460-39467.

Basak, C., Pathak, S.P., Bhattacharyya, A., Mandal, D., Pathak, S. and Kundu, M.(2005) NF-ķB and C/EBPβ-driven Interleukin-1β gene expression and PAK1-mediated Caspase-1 activation play essential roles in Interleukin-1β release from Helicobactor pylori Lipopolysaccharide-stimulated macrophages. J. Biol. Chem. 280(6), 4279-4288.

Pathak, S.K., Bhattacharyya, A., Pathak, S., Basak, C., Mandal, D., Kundu, M.and Basu, J. (2004). Toll-like receptor 2 and Mitogen- and stress- activated Kinase 1are effectors of Mycobacterium avium-induced Cyclooxygenase-2 expression in macrophages. J. Biol. Chem. 279 (53), 55127-55136.

Mandal, D., Baudin-Creuza, V., Bhattacharya, A., Pathak, S., Delauney, J., Kundu, M. and Basu, J. (2003) Caspase 3-mediated proteolysis of N-terminal cytoplasmic domain of human erythroid anion exchanger 1 (Band 3). J. Biol. Chem. 278 (52), 52551-52558.

Mandal, D., Moitra, P. K., Saha. S. and Basu, J. (2002) Caspase 3 regulates phosphatidylserine externalization and phagocytosis of oxidatively stressed erythrocytes. FEBS Letters, 513, 184-188.

Mandal, D., Moitra, P.K. and Basu, J. (2002) Mapping of a spectrin-binding domain of human erythrocyte membrane protein 4.2. Biochem.  J., 364, 841-847
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