Faculty Members

Dr. Amit Roy
Assistant Professor &
INSA Young Scientist

Department of Biotechnology

National Institute of Pharmaceutical Education & Research,
(NIPER), Hajipur-844 102, Bihar, India.
E-mail:amitavik@gmail.com


Ph.D Positions are Available:
Interested candidates, with individual fellowships (CSIR-NET-JRF/DBT-JRF/DST-Inspire/ICMR-JRF…etc.) and having good academic record, highly motivated & dedicated in Biological Science to pursue research in frontier areas of Molecular and Cellular Biology, are encouraged to apply for JRF position to amitavik@gmail.com
Educational Qualifications:
Ph.D (2009), Indian Institute of Chemical Biology (IICB), Jadavpur University, Kolkata
Master of Science (2002), University of Burdwan (1st Class)
Bachelor of Science (2000), University of Burdwan (1st Class)
Professional Experiences:
Assistant Professor (July 2015 to till date) at Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur, India
Senior Lecturer (February 2013 to July 2015) at Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur, India

Postdoctoral Research Fellow (February 2010 to January 2013) at Department of Molecular Biology and Genetics, University of Aarhus, Denmark

Area of Specialization:

Molecular Biology, Molecular Nanotechnology, Biochemistry, Enzymology, Molecular Parasitology, Pharmacology, Physiology, Cancer Stem Cells.

Current Area of Research:

DNA Topoisomerases, Drug Development and Therapeutics, Parasitology, Cancer Stem Cells

Awards and Recognitions:

INSA Young Scientist Award (2011) by Indian National Science Academy (INSA), Govt. of India, New Delhi, India.

Professor LSS Kumar Memorial Award (2011) by Indian National Science Academy (INSA), Govt. of India, New Delhi, India.

Certificate of Appreciation for Oral Presentation (2009), Selected out of 200 participants of Poster presentation in 78th Annual Meeting of “Society of Biological Chemists (SBC, India)”, Pune, India.

Best Poster Award (2008) in “US-India Joint Training Program Workshop on Intracellular Pathogens” at Indian School of Business (ISB), Hyderabad, organized jointly by Jawaharlal Nehru University (JNU), India; Seattle Biomedical Research Institute (SBRI), Seattle, USA and University of Washington, USA.

Best Presentation Award (2008) in “15th West Bengal State Science and Technology Congress” atShibpur BE College, Howrah, India.

Certificate of excellent performance in programme events during the year 2006-2007 for popularization of Science & Technology in Society by Science Association of Bengal, Kolkata, West Bengal, India.

Grants

Title of the Project

Funding Agency

Duration

Amount

Single molecule detection of human topoisomerase I dependent reactions at the single cell level: Unravelling cell-to-cell variability

Postdoctoral Grant,
Carlsberg Foundation, Copenhagen, Denmark

Two Years
(January 2011 to December 2012)

1,096,438 DKK = ~
Rs. 1.10 Crore

Role of HIV-1 protease inhibitors on catalytic activity of Leishmania donovani Topoisomerase I for the treatment of HIV-VL co-infection

INSA Young Scientist Grant, Indian National Science Academy,
Govt. of India,
New Delhi, India

Three Years
(November 2014 to October 2017)

Rs. 15 Lakhs

 

 

Research Publications:
22. Niels Frank Jensen1, Keli Agama, Amit Roy, David Hersi Smith, Thomas D. Pfister, Maria Unni Rømer, Hong-Liang Zhang, James H. Doroshow, Birgitta R. Knudsen, Jan Stenvang, Nils Brünne and Yves Pommier (2016) Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations. Journal of Experimental & Clinical Cancer Research. 35:56.
21.
Amit Roy, Cinzia Tesauro, Rikke Frøhlich, Marianne S. Hede, Maria J. Nielsen, Eigil Kjeldsen, Bjarne Bonven, Magnus Stougaard, Irina Gromova, Birgitta R. Knudsen (2014) Decreased Camptothecin Sensitivity of the Stem-Cell-like Fraction of Caco2 cells Correlates with an Altered Phosphorylation Pattern of Topoisomerase I. PLoS ONE. 9(6): e99628
20.
Joanna Proszek, Amit Roy, Ann-Katrine Jakobsen, Rikke Frøhlich, Birgitta R. Knudsen, Magnus Stougaard (2014) Topoisomerase I as a biomarker: Detection of activity at the single molecule level. Sensors, 14: 1195-1207.
19.
SisselJuul,Christine J. F. Nielsen,Rodrigo Labouriau, Amit Roy, CinziaTesauro, Pia W. Jensen, Emil L. Kristoffersen, Charlotte Harmsen, Ya-Ling Chiu, RikkeFrøhlich, Paola Fiorani, Janet Cox, David P. Tordrup, Jørn Koch, Anne-LiseBienvenu, Alessandro Desideri, Stephane Picot, Eskild Petersen, Kam W. Leong, Yi-Ping Ho, Magnus Stougaard, and Birgitta R. Knudsen (2012)A Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria Causing Plasmodium Parasites Based on Enzyme Activity Measurement. ACS Nano. 6(12): 10676–10683.
18.
Amit Roy, SayanChowdhury, SouvikSengupta, MadhumitaMandal, ParasuramanJaisankar, IldaD’Annessa, Alessandro Desideri and Hemanta K. Majumder (2011) Development of derivatives of 3, 3’-Diindolylmethane as potent Leishmaniadonovani bi-subunit topoisomerase IB poisons. PLoS ONE.6(12): e28493.
17.
Amit Roy, IldaD’Annessa, Christine J. F. Nielsen, David Tordrup, Rune R. Laursen,Birgitta Ruth Knudsen, Alessandro Desideri, and Felicie Faucon Andersen(2011) Peptide Inhibition of Topoisomerase IB from Plasmodium falciparum.Molecular Biology International. Volume 2011, Article ID 854626, 10 pages.
16.
Souvik Sengupta, Agneyo Ganguly, Amit Roy, Somdeb Bose Das gupta, IldaD’Annessa, Alessandro Desideri, Hemanta K. Majumder. (2011) ATP independent type IB topoisomerase of Leishmaniadonovani is stimulated by ATP: an insight into the functional mechanism.Nucleic Acids Res. 39 (8): 3295-3309.
15.
Bijoylaxmi Banerjee, Amit Roy, Nilkantha Sen, and Hemanta K. Majumder (2010) A tyrosyl DNA phosphodiesterase 1 from kinetoplastid parasite Leishmaniadonovani (LdTdp1) capable of removing topo I-DNA covalent complexes. Mol Microbiol. 78(1): 119-37
14.
Agneyo Ganguly, Souvik Sengupta, Somdeb BoseDasgupta, Amit Roy, Hemanta K. Majumder(2009) Mutational studies reveal lysine 352 on the large subunit is indispensable for catalytic activity of bi-subunit topoisomerase I from Leishmania donovani. Molecular & Biochemical Parasitology. 165: 57–66.
13.
Amit Roy, Somdeb Bose Dasgupta, Agneyo Ganguly, Parasuraman Jaisankar, and Hemanta K. Majumder. (2009) Topoisomerase I gene mutations at F270 in the large subunit and at N184 in the small subunit contribute to the resistance mechanism of unicellular parasite Leishmaniadonovani towards 3,3'-Diindolylmethane (DIM). Antimicrob. Agents Chemother.53(6): 2589-2598.
12.
Amit Roy, Agneyo Ganguly, SomdebBosedasgupta, Ben uBrata Das, Churala Pal, Parasuraman Jaisankar and Hemanta K. Majumder (2008) Mitochondria dependent ROS-mediated programmed cell death (PCD) induced by 3,3'-Diindolylmethane (DIM) through Inhibition of FoF1-ATP synthase in unicellular protozoan parasite Leishmania donovani. Mo lPharmacol. 74 (5): 1292-1307.
11.
Amit Roy, Benu Brata Das, Agneyo Ganguly, Somdeb BoseDasgupta, Neeta VM. Khalkho, Churala Pal, Sumit Dey, VenkatachalamSeshaGiri, Parasuraman Jaisankar, Sanjit Dey and Hemanta K. Majumder (2008) An insight into the mechanism of inhibition of unusual bi-subunit topoisomerase I from Leishmaniadonovani by 3,3'-diindolylmethane, a novel DNA topoisomerase I poison with a strong binding affinity to enzyme. Biochem J.,409(2): 611-622.
10.
Swapan Pramanick, Amit Roy, Hemanta K. Majumder, Shekher Ghosh and Sibabrata Mukhopadhyay. (2008) Withanolide Z, a New Chlorinated Withanolide from Withaniasomnifera. PlantaMedica. 74 (14): 1745-8.
09.
Somdeb BoseDasgupta, Benu Brata Das, SouvikSengupta, Agneyo Ganguly, Amit Roy, S Dey, Gayatri Tripathi, Biswanath Dinda and Hemanta K. Majumder (2008) Thecaspase-independent algorithm of programmed cell death in Leishmania induced by baicalein: the role of LdEndoG, LdFEN-1 and LdTatD as a DNA 'degradesome'. Cell Death Differ. 15(10): 1629-40.
08.
Rakhee Das, Amit Roy, Neeta Dutta and Hemanta K. Majumder (2008) Reactive oxygen species and imbalance of calcium homeostasis contributes to curcumin induced programmed cell death in Leishmania donovani. Apoptosis. 13(7): 867-82.
07.
Somdeb BoseDasgupta, Benu Brata Das, Souvik Sengupta, Agneyo Ganguly, Amit Roy, Gayatri Tripathi and Hemanta K. Majumder. (2008) Amino acids 39-456 of large subunit and 210-262 of small subunit constitute the minimal, functionally interacting fragments of the unusual heterodimeric topoisomerase IB of Leishmania. Biochem J., 409(2):481-489.
06.
Somdeb BoseDasgupta,Agneyo Ganguly, Amit Roy, Tanmoy Mukherjee and Hemanta K. Majumder (2008) A novel ATP-binding cassette transporter, ABCG6 is involved in chemoresistance of Leishmania. MolBiochem Parasitol. 158(2):176-188.
05.
Somdeb BoseDasgupta, Agneyo Ganguly, Benu Brata Das, Amit Roy, Neeta V. M. Khalkho and Hemanta K. Majumder (2008) The large subunit of Leishmaniatopoisomerase I functions as the ‘molecular steer’ in type IB topoisomerase. Molecular Microbiology, 67(1):31-46.

04.
Agneyo Ganguly, Benu brata Das, Amit Roy, Nilkantha Sen, Somdeb BoseDasgupta, Shibabrata Mukhopadhayay and Hemanta K. Majumder (2007)Betulinic acid, a catalytic inhibitor of topoisomerase I inhibits ROS mediated apoptotic topo I-DNA cleavable complex formation in prostate cancer cells but does not affect the process of cell death. Cancer Res., 67(24):11848-11858.
03.
Benu Brata Das, Somdeb BoseDasgupta, Agneyo Ganguly, Saumyabrata Mazumder, Amit Roy and Hemanta K. Majumder (2007) Leishmania donovani bisubonit topoisomerase I gene fusion leads to an active enzyme with conserved type IB enzyme function. FEBS J.,274: 150-163.
02.
Agneyo Ganguly, Benu Brata Das, Nilkantha Sen, Amit Roy, Somdeb BoseDasgupta and Hemanta K. Majumder (2006) 'LeishMan' topoisomerase I: an ideal chimera for unraveling the role of the small subunit of unusual bi-subunit topoisomerase I from Leishmaniadonovani. Nucleic Acids Res., 34: 6286-6297

01.
Benu Brata Das, Nilkantha Sen, Amit Roy, Somdeb BoseDasgupta, Agneyo Ganguly, Bikash Chandra Mohanta, Biswanath Dinda and Hemanta K. Majumder (2006) Differential induction of Leishmaniadonovani bi-subunit topoisomerase I-DNA cleavage complex by selected flavones and camptothecin: activity of flavones against camptothecin-resistant topoisomerase I. Nucleic Acids Res., 34: 1121-1132.
Book Chapter:

“Physiology Update” Vol. 1 (2009): 124-140.

“Physiology Update” Vol. 2 (2010): 81-96.
Membership of Professional Societies:
01.

Life Member (Membership No: 3379) at Society Of Biological Chemists(I), New Biological Science Building D- Wing, 1st Floor, Indian Institute of Science, Bangalore-560 012, INDIA.


02.
Life Member (Membership No: L25734) at Indian Science Congress Association, 14, Dr. Biresh Guha Street, Kolkata-700017, West Bengal, INDIA.
Invited Talks in India and Abroad:
9.
Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, India on 2nd March, 2016 for collaboration development.
8.
6th World Congress on Biotechnology held at Crowne Plaza, New Delhi, India during October 05-07, 2015; organized by OMICS International group.
7.
Department of Biological Sciences (DBS), Indian Institute of Science Education and Research (IISER), Mohanpur Campus, Mohanpur - 741 252, West Bengal, India on 16th January 2014, entitled “DNA Topoisomerase I and Therapeutic Approaches”.
6.
Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, 784028, Assam, India on 13th February 2013, entitled “A Journey with DNA Topoisomerase I”.
5.
Department of Oncogene Regulation and Viral associated Human cancer, Chittaranjan National Cancer Institute, 37, S.P.Mukherjee Road, Kolkata-700026, West Bengal, India on 16th August 2012, entitled “Putative differences between cancer stem cells and progeny cells with respect to hTopI activity and CPT sensitivity”.
4.
Rajendra Memorial research Institute of Medical Sciences (ICMR), Agamkuan, Patna 800 007, Bihar, India on 14th August 2012, entitled “A Journey with Topoisomerase I”.
3.
Department of Biotechnology, University of Burdwan, West Bengal, India on 5th January 2012, entitled “Topoisomerase I and pharmacology”.
2.
Laboratory of Genome Research, Department of Molecular Biology, Aarhus University. C. F. Mollers Allé, Bldg. 1130, DK-8000 Aarhus C, Denmark on 2nd Sepetember 2009, entitled “DIM and DNA Topoisomerase I: Therapy and Resistance”.
1.
Department of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland on 4th June 2009, entitled “A Journey with DIM, an inhibitor of DNA Topoisomerase IB of Leishmania”.
Country Visited:

Country

Institute/University

Date

Duration

Purpose of Visit

Switzerland

Dept. of Biochemistry,
Biozentrum,
University of Basel

June 2009

10 Days

Invited Lecture and Visiting Fellow

Denmark

Dept. of Molecular Biology
and Genetics,
Aarhus University

September 2009

4 Days

Invited Lecture and Visiting Fellow

Denmark

Dept. of Molecular Biology
and Genetics,
Aarhus University

February 2010 to
January 2013

3 Years

Postdoctoral Research Fellow

Germany

Hannover Exhibition Grounds, Deutsche Messe,
Hannover, Germany

October 2011

3 Days

Conference

Conferences Attended in India and Abroad:
19.
Two days’ workshop and training on Good Clinical Laboratory Practices (GCLP) held on 22nd to 23rd June, 2016 sponsored by DNDi at Rajendra Memorial Research Institute of Medical Sciences (ICMR), Agamkuan, Patna-800007, Bihar, India.
18.
Two days’ workshop on Intellectual Property Rights (IPR) from 15th to 16th March, 2016 sponsored by IPR Unit, ICMR, Department of Health & Research, Govt. of India, New Delhi at Rajendra Memorial Research Institute of Medical Sciences (ICMR), Agamkuan, Patna-800007, Bihar, India.
17.
IMMUNOCON-2015, 42nd Annual Conference of Indian Immunology Society held on October 09-11, 2015; organized by Rajendra Memorial Research Institute of Medical Sciences (RMRIMS), Patna, India.
16.
6th World Congress on Biotechnology held at Crowne Plaza, New Delhi, India during October 05-07, 2015; organized by OMICS International group
15.
83rd Annual Meeting of “Society of Biological Chemists (India)” held on 83rd Annual Meeting of “Society of Biological Chemists (India)” held on December 18 to December 21, 2014; organized by KIIT University, Bhubaneswar, Institute of Life Science, Bhubaneswar and National Institute of Science Education and Research (NISER), Bhubaneswar, India.
14.
“Molecular Diagnostics Summit Europe” held on October 11 - 13, 2011 at convencence center, Exhibition ground, Hannover, Germany organized by Cambridge Healthtech Institute.
13.
“Indo-Brazil Symposium on Infectious Diseases-2009” held on December 10-11, 2009, organized by Indian Institute of Chemical Biology (CSIR) and Jawaharlal Nehru Centre for Advanced Scientific Research at Hotel Floatel, Strand Road, Kolkata-700 001, India.
12.
78th Annual Meeting of “Society of Biological Chemists (India)” held on Oct 30 to Nov 1, 2009 at YASHADA, Raj Bhavan Complex, Baner Road, Pune- 411007, India organized by National center for cell science, and University of Pune, Pune-411007, India.
11.
International symposium on System Biology and Proteomics in Biomedical Science held on 27th to 29th March 2009 at Vedic Village, Kolkata, India, organized by Indian Institute of Chemical Biology, Kolkata, India.
10.
16th West Bengal State Science and Technology Congress held on 28th February to 1st March 2009 at Burdwan University, Burdwan, West Bengal, India, organized by West Bengal Council of Science and Technology, West Bengal, India.
9.
4thWorldLeish Congress on Leishmaniasis 2009 (WorldLeish4) held on February 03-07, 2009 at Scientific Convention Center, Lucknow, India organized by Central Drug Research Institute (CDRI), Lucknow, India.
8.
Symposium on Recent Advances in Biological Sciences, Society of Biological Chemistry (India) Kolkata Chapter, held on August 22 to 24, 2008 at Digha, West Bengal, India.
7.
Acharya Prafulla Chandra Ray Memorial Symposiun on Chemistry Today (2008) held on 01-02 August 2008 at Central Glass and Ceramic Research Institute (CGCRI), Kolkata, India, organized by The Indian Chemical Society, Kolkata, India.
6.
15th West Bengal State Science and Technology Congress held on 28-29 February 2008, at Shibpur, Howrah, West Bengal, India, organized by West Bengal Council of Science and Technology, West Bengal, India.
5.
US-India Joint Training Program Workshop on Intracellular Pathogens held on 21-24 January 2008 at Indian School of Business (ISB), Gachibowli, Hyderabad-500 032, India organized jointly by Jawaharlal Nehru University, New Delhi, India; Seattle Biomedical Research Institute (SBRI), Seattle; and University of Washington, USA.
4.
Symposium on Natural Products: Past, Present and Future (NPPPF-2007) held on August 3, 2007, organized Prof. R. N. Chakravarti 90th Birth Anniversary Celebration Committee at Central Glass and Ceramic Research Institute (CGCRI), Kolkata, India.
3.
International Symposium on Chemical Biology, Golden Jubilee Celebration (1956-2006) held on March 7-9, 2007, organized by Indian Institute of Chemical Biology (IICB), Kolkata, India.
2.
18th National Congress of Parasitology on Advances in Parasitology Research of Tropical Diseases held on November 22-24, 2006 at Indian Institute of Chemical Biology, Kolkata, India organized by Indian Institute of Chemical Biology and Indian Society of Parasitology, India.
1.
Drug Initiatives in Leishmaniasis (ICMR sponsored symposium) held on 9th February, 2005 at Department of Pharmacology, Institute of Postgraduate Medical Education and Research (IPGMER), Kolkata, India, organized by Department of Pharmacology, Institute of Postgraduate Medical Education and Research (IPGMER), Kolkata, India.
Talks at National/International Meeting:

Presented the work entitled “Topoisomerase I amino acid substitutions, Phy270Leu in the large subunit and Asp184Ser in the small subunit, confer 3,3'-Diindolylmethane (DIM)-resistance in Leishmaniadonovani” in 78th Annual Meeting of “Society of Biological Chemists (India)” on Oct 30 to Nov 1, 2009 organized by National Center for Cell Science (NCCS) and University of Pune at Pune, India.(Selected Presentation)

Presented the work entitled “Mechanism of action of the three derivatives of potent anti-leishmanial agent 3, 3'-Diindolylmethane (DIM)” in “16th West Bengal State Science and Technology Congress” on 28th February to 1st March 2009 at Burdwan University, India.

Presented the work entitled “ Inhibition of mitochondrial F0F1-ATP synthase contributes to DIM-induced PCD in unicellular protozoan parasite Leishmania donovani” at Society of Biological Chemistry (India) Kolkata Chapter on August 22 to 24, 2008 at Digha, India.

Presented the work entitled “3,3'-Diindolylmethane induced Programmed Cell Death through Mitochondria dependent signaling pathways in unicellular protozoan parasite Leishmaniadonovani” in “15th West Bengal State Science and Technology Congress” on 28th February to 29th February 2008 at Shibpur BE College, Howrah, India. (Best Presentation Awarded)

Poster Presentation:
Presented poster entitled “Hypophosphorylation of DNA Topoisomerase I Confers Campothecin Resistance of the Cancer Stem-Like cell Population of Caco2 Cell Line” at 83rd Annual Meeting of “Society of Biological Chemists (India)” held on December 18 to December 21, 2014; organized by KIIT University, Bhubaneswar, Institute of Life Science, Bhubaneswar and National Institute of Science Education and Research (NISER), Bhubaneswar, India. [Poster No. P-14-13]

Presented poster entitled “Rapid and Reliable Malaria Diagnosis: Novel Plasmodium Topoisomerase I Specific Nucleotide Biosensor assay as diagnostic tools” at Molecular Diagnostics Summit Europe,organized by Cambridge Healthtech Institute, USA on October 11 - 13, 2011at Hannover,Germany.

Presented poster entitled “Topoisomerase I amino acid substitutions, Phy270Leu in the large subunit and Asp184Ser in the small subunit, confer 3,3'-Diindolylmethane (DIM)-resistance in Leishmaniadonovani” in 78th Annual Meeting of “Society of Biological Chemists (SBC, India)” on Oct 30 to Nov 1, 2009 organized by National Center for Cell Science (NCCS) and University of Pune at Pune, India. [Poster No. 22]

Presented poster entitled “F270 of Large subunit and N184 of Small subunit of L.donovani type IB topoisomerase confer resistance to Topoisomerase I poison 3,3'-Diindolylmethane (DIM)” in 4th WorldLeish Congress on Leishmaniasis 2009 (WorldLeish4) on 03-07 February 2009, organized by Central Drug Research Institute (CDRI) at Lucknow, India. [Poster No. 54]

Presented poster entitled “The mechanism of inhibition of unusual bi-subunit topoisomerase I from Leishmaniadonovani by 3,3'-Diindolylmethane, a Novel DNA Topoisomerase I poison” in the US-India Joint Training Program Workshop on Intracellular Pathogens on 21-24 January 2008at Indian School of Business (ISB), Hyderabad,India, organized jointly by Jawaharlal Nehru University (JNU), India; Seattle Biomedical Research Institute (SBRI), Seattle, USA and University of Washington, USA. (Best Poster Awarded)
 
     
     
     
 
     
   
       
 
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